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58问答库 > western-blot试验后得到了样本的灰度值，请问怎么算出样本蛋白的实际含量

western-blot试验后得到了样本的灰度值，请问怎么算出样本蛋白的实际含量

2025-04-02 16:32:44

推荐回答（1个）

回答（1）：

Western-blot是一种半定量的检测手段。
个人认为如果要通过灰度值来计算蛋白浓度，那就是和内参来做对比，因为内参的浓度是已知且单一条带的蛋白样品。通过待测样品盒内参样品灰度值做对比可以算出待测样品大概的浓度。
个人意见，仅供参考，因为一般检测蛋白含量的做法都是通过BF，BCA，Lowry之类的检测方法来测的。

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