我们实验室一般加引物都是上下游都是1ul的
10uM的浓度加0.5ul就好了
The important is not the volume of the primers, but the final primer concentration. The concentrations of both primers in the amplification reaction should be between 0.1 and 0.5 μM.
上下游都是0.5ul
这个要看你的引物的浓度