推荐回答(1个)
(一)细胞学研究
Lagercranz(1998)通过对拟南芥与黑芥连锁作图分析发现:芸薹属植物的二倍体种起源于一个原始六倍体种。Warwick(1999)用同工酶分析发现:在染色体基数大的种群中非整倍体及其细胞内染色体间的融合与分离比其多倍体在进化过程中所起的作用大。依据芸薹属植物在细胞减数分裂粗线期染色体间联会时所发生的优先配对现象的核型及系统发育分析发现:芸薹属芜菁种的染色体组成为AABCDDEFFF(即3个二体、2个四体、1个六体)。Habib Akmad(2004)通过秋水仙素处理芜菁形成多倍体后发现:46.7%多倍体后代有回复变成二倍体的现象,它们在减数分裂形成配子时,染色体发生不均衡分离,形成活力不同的非正常配子,这些非正常配子的联合作用在当今芸薹属种的进化过程中起着很重要的作用。
(二)分子生物学研究
在分子生物学研究方面,姚祥坦(2004)用抗TuMV的芜菁自交系耐病98-1与对TuMV极其敏感的芜菁品种温州盘菜为试材构建F2抗性分离群体,最后得到了一些与抗TuMV基因相关的AFLP条带。许志茹(2005)用光敏感型津田芜菁与光非敏感型赤丸芜菁为试材构建了2个cDNA文库,同时结合遮光处理与光照处理构建了4个消减文库,从中筛选出PAL、CHS、F3H、DFR、ANS等5个花青素生物合成途程所需的酶的结构基因,bHLH、WD40、MYB和bZIP等4个与花青素生物合成相关的调控因子的编码序列。并发现光敏感型津田芜菁在光照下所表达的基因数量多于在黑暗下所表达的基因数量,而赤丸芜菁在黑暗下所表达的基因数量多于津田芜菁在黑暗下所表达的基因数量;光敏感型津田芜菁块根根皮中花青素含量与光照时间呈正相关关系,而非光敏感型赤丸芜菁块根根皮中花青素的含量与光照时间没有明显的相关关系。
许志茹等(2006)以“津田”芜菁红色块根和白色块根为材料,利用SSH技术成功构建了消减cDNA文库并富集相关基因片段。通过对消减文库的筛选、鉴定、克隆测序和同源性比较,获得了特异基因片段302个;其中与数据库中的序列具有相似区域且功能已知的序列为99个,与数据库中序列没有显著同源性且功能未知的序列为203个。许志茹等(2006)又利用UV-A处理48h后津田芜菁块根变红,以黑暗处理条件下的白色块根为对照,与削减文库特异基因片段制备的cDNA微阵列进行杂交。UV-A处理条件下津田芜菁中表达上调的基因为81个,表达下调的基因为47个,表达上调的基因中包括与花青素生物合成直接相关的基因片段cytochromeP450、PAL、F3H、ANS、CHS、DFR和GST等。Northern杂交结果显示,UV-A处理48h的津田芜菁试材中,PAL、CHS、F3H、DFR和ANS基因的表达量明显高于黑暗条件下白色块根中这些基因的表达量,这进一步验证了芯片杂交结果的可靠性。
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