（安捷伦高效液相色谱仪）如何解决色谱柱使用过程中出现的问题—高效液相色谱柱
推荐回答（1个）
问题 原因 表现 不同色谱柱间差异 填料、键合相不同 保留因子（k），分离因子（α）
使用期间柱变化 柱床破坏 柱效（N）
键合相丢失 保留因子（k），分离子（α）
硅胶基质溶解 柱效（N）
强保留分堆积堵塞 保留因子（k），柱效（N） 柱外效应 系统差异，进样量大、 柱效（N）
进样阀与 色谱柱之间、
色谱柱与检测器之间管
路太长、检测器流通池
体积大、接头死体积大
流速改变 保留因子（k），分离因子（α）温度改变 保留因子（k），柱效变化很小 柱平衡慢 重新平衡时间不够 保留因子（k），柱效变化很小 柱超载 样品量太大 保留因子（k），柱效（N）
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